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Phenol Red Carbohydrate Broth with NaCl (BAM M121)

[所属分类:培养基配方] [发布时间:2021-12-9] [发布人:网站管理员2] [阅读次数:] [返回]

Phenol Red Carbohydrate Broth

with NaCl

(BAM M121)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl..................................................................30.0g

Pancreatic digest of casein................................10.0g

Beef extract.........................................................1.0g

Carbohydrate solution................................. 200.0mL

Phenol Red solution........................................ 7.2mL

pH 7.4 ± 0.2 at 25°C

Source: This medium without added NaCl is avail

able as a premixed powder from BD Diagnostics.

Phenol Red Solution:

Composition per 10.0mL:

Phenol Red......................................................0.025g

Preparation of Phenol Red Solution: Add Phe

nol Red to distilled/deionized water and bring vol

ume to 10.0mL. Mix thoroughly.

Carbohydrate Solution:

Composition per 200.0mL:

Carbohydrate..............................................5.0–10.0g

Preparation of Carbohydrate Solution: Add

carbohydrate (5.0g dulcitol, 10.0g lactose, or 10.0g

sucrose) to distilled/deionized water and bring vol

ume to 200.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept carbohydrate solution, to distilled/deionized wa

ter and bring volume to 800.0mL. Mix thoroughly.

Adjust pH to 7.4 if necessary. Distribute into tubes

containing an inverted Durham tube. Fill each tube

with 8.0mL of medium. Autoclave for 15 min at 13 psi

pressure–118°C. Cool to 45°–50°C. Aseptically add

2.0mL of sterile carbohydrate solution to each tube.

Use: For the determination of fermentation reactions

in the differentiation of halophilic Vibrio spp. Fer

mentation is determined by the production of acid—

broth turns yellow—and formation of gas—bubble

trapped in Durham tube.