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Peptone Water with Andrade’s Indicator

[所属分类:培养基配方] [发布时间:2021-12-8] [发布人:网站管理员2] [阅读次数:] [返回]

Peptone Water with Andrade’s

Indicator

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Peptone..............................................................10.0g

NaCl....................................................................5.0g

Andrade’s indicator..................................... 100.0mL

Carbohydrate solution................................... 20.0mL

pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from Oxoid.

Andrade’s Indicator:

Composition per 100.0mL:

Acid Fuchsin.......................................................0.1g

NaOH (1N solution)...................................... 16.0mL

Caution: Acid Fuchsin is a potential carcinogen

and care must be taken to avoid inhalation of the

powdered dye and contact with the skin.

Carbohydrate Solution:

Composition per 20.0mL:

Carbohydrate..............................................5.0–10.0g

Preparation of Carbohydrate Solution: Add

carbohydrate to distilled/deionized water and bring

volume to 20.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept carbohydrate solution, to distilled/deionized wa

ter and bring volume to 980.0mL. Mix thoroughly.

Adjust pH to 7.4 if necessary. Distribute into tubes

containing an inverted Durham tube. Fill each tube

with 9.8mL of medium. Autoclave for 15 min at 15

psi pressure–121°C. Aseptically add 0.2mL of sterile

carbohydrate solution to each tube.

Use: For use in carbohydrate fermentation tests. Fer

mentation is determined by the production of acid—

broth turns pink—and formation of gas—bubble

trapped in Durham tube.