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Oatmeal Agar A (ISP-3) (DSMZ Medium 609)

[所属分类:培养基配方] [发布时间:2021-12-7] [发布人:网站管理员2] [阅读次数:] [返回]

Oatmeal Agar A

(ISP-3)

(DSMZ Medium 609)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Oatmeal (Quaker White Oats) ..........................20.0g

Agar ..................................................................18.0g

Trace salts solution ......................................... 1.0mL

pH 7.0 ± 0.2 at 25°C

Trace Salt Solution:

Composition per 100mL:

FeSO4·7H2O........................................................0.1g

MnCl2·4H2O .......................................................0.1g

ZnSO4·7H2O .......................................................0.1g

Preparation of Trace Salt Solution: Add com

ponents to 100.0mL distilled/deionized water. Mix

thoroughly.

Preparation of Medium: Add oats to 1.0L dis

tilled/deionized water. Adjust pH to 7.2. Gently heat

and bring to boiling. Cook or steam for 20 min. Filter

through cheese cloth. Add 18.0g agar. Add 1.0mL

trace salt solution. Bring volume to 1.0L distilled/

deionized water. Gently heat and bring to boiling.

Autoclave for 15 min at 15 psi pressure–121°C. Pour

into Petri dishes or distribute to sterile tubes.

Use: For the cultivation of various fungi.