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Nitrate Broth, Enriched (BAM M109)

[所属分类:培养基配方] [发布时间:2021-12-6] [发布人:网站管理员2] [阅读次数:] [返回]

Nitrate Broth, Enriched

(BAM M109)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein................................13.0g

NaCl....................................................................5.0g

Yeast extract........................................................5.0g

Heart muscle, solids from infusion .....................2.0g

KNO3 ..................................................................2.0g

pH 7.3 ± 0.2 at 25°C

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Distribute into test tubes that con

tain an inverted Durham tube. Autoclave for 15 min

at 15 psi pressure–121°C.

Use: For the differentiation of aerobic and faculta

tive Gram-negative microorganisms based on their

ability to reduce nitrate to nitrite or form N2 gas. Test

for nitrates with sulfanilic acid and α-naphthylamine

reagents. Bacteria that reduce nitrate to nitrite turn

the reagents red or pink.