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Nitrate Broth (International Streptomyces Project Medium 8) (ISP Medium 8) (ATCC Medium 872) (BAM M108)

[所属分类:培养基配方] [发布时间:2021-12-6] [发布人:网站管理员2] [阅读次数:] [返回]

Nitrate Broth

(International Streptomyces

Project Medium 8)

(ISP Medium 8)

(ATCC Medium 872)

(BAM M108)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Peptone................................................................5.0g

Beef extract.........................................................3.0g

KNO3 ..................................................................1.0g

pH 7.0 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Distribute into tubes or flasks. Auto

clave for 15 min at 15 psi pressure–121°C.

Use: For the differentiation of aerobic and facultative

Gram-negative microorganisms based on their ability to

reduce nitrate. Test for nitrates with sulfanilic acid and

α-naphthylamine reagents. Bacteria that reduce nitrate

to nitrite turn the reagents red or pink.