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Nickels and Leesment Agar, Modified

[所属分类:培养基配方] [发布时间:2021-12-6] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Part 1........................................................... 750.0mL

Part 2........................................................... 100.0mL

Part 3........................................................... 100.0mL

Part 4............................................................. 50.0mL

pH 6.65 ± 0.2 at 25°C

Part 1:

Composition per 750.0mL:

Pancreatic digest of casein................................20.0g

Lactose..............................................................10.0g

Yeast extract........................................................5.0g

NaCl....................................................................4.0g

Gelatin.................................................................2.5g

Sodium citrate.....................................................2.0g

Preparation of Part 1: Add components to dis

tilled/deionized water and bring volume to 750.0mL.

Mix thoroughly. Adjust pH to 6.65.

Part 2:

Composition per 500.0mL:

Nonfat dry milk.................................................50.0g

Preparation of Part 2: Add nonfat dry milk to

distilled/deionized water and bring volume to

500.0mL. Inoculate with Lactobacillus bulgaricus.

Incubate at 20°C for 24 hr. Centrifuge at 5000 rpm

for 10 min to separate the curd. Collect the superna

tant solution. Autoclave for 15 min at 15 psi pres

sure–121°C. Store at 4°C.

Part 3:

Composition per 100.0mL:

Calcium citrate..................................................13.3g

Carboxymethylcellulose .....................................0.8g

Preparation of Part 3: Combine the calcium cit

rate and carboxymethylcellulose in a mortar and

grind until a fine powder. Add powder to 100.0mL of

hot distilled/deionized water. Mix thoroughly. Filter

through cheesecloth.

Part 4:

Composition per 50.0mL:

Calcium lactate ...................................................8.0g

Preparation of Part 4: Add calcium lactate to dis

tilled/deionized water and bring volume to 50.0mL.

Mix thoroughly. Gently heat until dissolved.

Preparation of Medium: Prepare each of the four

parts separately. Autoclave each part for 15 min at 15

psi pressure–121°C. Aseptically combine part 1, part

2, part 3, and part 4. Mix thoroughly. Pour into sterile

Petri dishes. Swirl flask while dispensing medium.

Use: For the isolation and cultivation of acid-pro

ducing microorganisms from foods.

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Nickels and Leesment Agar, Modified