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Neopeptone Glucose Rose Bengal Aureomycin® Agar

[所属分类:培养基配方] [发布时间:2021-12-6] [发布人:网站管理员2] [阅读次数:] [返回]

Neopeptone Glucose Rose Bengal

Aureomycin® Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................20.0g

Neopeptone.........................................................5.0g

Glucose ...............................................................1.0g

Tetracycline solution....................................... 5.0mL

Rose Bengal solution ...................................... 3.5mL

pH 6.5 ± 0.2 at 25°C

Tetracycline Solution:

Composition per 150.0mL:

Tetracycline.........................................................1.0g

Preparation of Tetracycline Solution: Add tet

racycline to distilled/deionized water and bring vol

ume to 150.0mL. Mix thoroughly. Filter sterilize.

Rose Bengal Solution:

Composition per 100.0mL:

Rose Bengal ........................................................1.0g

Preparation of Rose Bengal Solution: Add

Rose Bengal to distilled/deionized water and bring

volume to 100.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept tetracycline solution, to distilled/deionized wa

ter and bring volume to 995.0mL. Mix thoroughly.

Gently heat and bring to boiling. Autoclave for 15

min at 15 psi pressure–121°C. Cool to 45°–50°C.

Aseptically add 5.0mL of sterile tetracycline solu

tion. Mix thoroughly. Pour into sterile Petri dishes or

distribute into sterile tubes.

Use: For the isolation and cultivation of a wide vari

ety of fungal species.