Antibiotic Assay Medium No. 34

[所属分类:培养基配方] [发布时间:2019-10-22] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Antibiotic Assay Medium No. 34
Composition per liter:
Peptone........................................................................................10.0g
Beef extract.................................................................................10.0g
Glycerol ......................................................................................10.0g
NaCl..............................................................................................3.0g
pH 7.0 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes.
Use: For antibiotic assay effectiveness testing of bleomycin using
Mycobacterium smegmatis ATCC 607.