Antibiotic Assay Medium No. 19

[所属分类:培养基配方] [发布时间:2019-10-22] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Antibiotic Assay Medium No. 19
Agar............................................................................................23.5g
Glucose.......................................................................................10.0g
NaCl............................................................................................10.0g
Peptone .........................................................................................9.4g
Yeast extract..................................................................................4.7g
Beef extract...................................................................................2.4g
pH 6.1 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes.
Use:  For assaying the mycostatic activity of pharmaceutical prepara-
tions. For seed agar for the plate assay to test the effectiveness of nys-
tatin, amphotericin B, and natamycin.