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培养基配方

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m-CP Medium

[所属分类:培养基配方] [发布时间:2021-12-1] [发布人:网站管理员2] [阅读次数:] [返回]

m-CP Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Tryptose ............................................................30.0g

Yeast extract......................................................20.0g

Agar ..................................................................15.0g

Sucrose................................................................5.0g

L-Cysteine·HCl·H2O ...........................................1.0g

MgO4·7H2O ........................................................0.1g

Bromcresol Purple ............................................0.04g

Phenolphthalein biphosphate tetrazolium

salt solution............................................. 10.0mL

Selective supplement solution ........................ 4.0mL

Indoxyl-β-D-glucoside solution ...................... 4.0mL

Ferric chloride solution................................... 1.0mL

pH 7.6 ± 0.2 at 25°C

Selective Supplement Solution:

Composition per 4.0mL:

D-Cycloserine......................................................0.4g

Polymyxin B sulfate.......................................25.0mg

Preparation of Selective Supplement Solu

tion: Add components to 4.0mL of distilled/deion

ized water. Mix thoroughly. Filter sterilize.

Phenolphthalein Biphosphate Tetrazolium

Salt Solution:

Composition per 10.0mL:

Phenolphthalein biphosphate tetrazolium

salt ...........................................................25.0mg

Preparation of Phenolphthalein Biphosphate

Tetrazolium Salt Solution: Add phenolphthalein

biphosphate tetrazolium salt to 10.0mL of distilled/

deionized water. Mix thoroughly. Filter sterilize.

Indoxyl-β-D-glucoside Solution:

Composition per 10.0mL:

Indoxyl-β-D-glucoside ......................................0.45g

Preparation of Indoxyl-β-D-glucoside Solu

tion: Add indoxyl-β-D-glucoside to 10.0mL of dis

tilled/deionized water. Mix thoroughly. Filter

sterilize.

Ferric Chloride Solution:

Composition per 4.0mL:

FeCl3·6H2O....................................................30.0mg

Preparation of Ferric Chloride Solution: Add

FeCl3·6H2O to 4.0mL of distilled/deionized water.

Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components ex

cept selective supplement solution, phenolphthalein

biphosphate tetrazolium salt solution, indoxyl-β-D

glucoside solution, and ferric chloride solution, to

distilled/deionized water and bring volume to

981.0mL. Mix thoroughly. Autoclave for 15 min at

15 psi pressure–121°C. Cool to 45°–50°C. Aseptical

ly add 4.0mL of selective supplement solution. Mix

thoroughly. Aseptically add 10.0mL phenolphthalein

biphosphate tetrazolium salt solution, 4.0mL indox

yl-β-D-glucoside solution, and 1.0mL ferric chloride

solution. Mix thoroughly. Pour into sterile Petri dishes

or aseptically distribute into tubes.

Use: A selective, chromogenic medium for the rapid

identification and enumeration of Clostridium per

fringens in water samples, including water used in

food and beverage production.

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m-CP Medium