Antibiotic Assay Medium No. 8 (Base Agar with low pH)

[所属分类:培养基配方] [发布时间:2019-10-22] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Antibiotic Assay Medium No. 8
(Base Agar with low pH)
Composition per liter:
Agar............................................................................................15.0g
Peptone .........................................................................................6.0g
Yeast extract..................................................................................3.0g
Beef extract...................................................................................1.5g
pH 5.9 ± 0.1 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes.
Use: For antibiotic assay testing. For use as the base agar and the seed
agar in the plate assay of tetracycline. For use as the seed agar in the
plate assay of vancomycin, mitomycin, and mithramycin.