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MacConkey Agar, CS (BAM M91)

[所属分类:培养基配方] [发布时间:2021-11-30] [发布人:网站管理员2] [阅读次数:] [返回]

MacConkey Agar, CS

(BAM M91)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Peptone .............................................................17.0g

Agar ..................................................................13.5g

Lactose..............................................................10.0g

NaCl....................................................................5.0g

Proteose peptone.................................................3.0g

Bile salts..............................................................1.5g

Neutral Red.......................................................0.03g

Crystal Violet...................................................1.0mg

pH 7.1 ± 0.2 at 25°C

Source: This medium is available as a prepared me

dium from BD Diagnostic Systems.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat while stirring until boil

ing. Autoclave for 15 min at 15 psi pressure–121°C.

Pour into sterile Petri dishes or distribute into sterile

tubes.

Use: For the cultivation and differentiation of lac

tose-fermenting and nonfermenting Gram-negative

bacteria while also controlling the swarming of Pro

teus species, if present. Lactose-fermenting organ

isms appear as red to pink colonies. Lactose-nonfer

menting organisms appear as colorless or transparent

colonies.