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MacConkey Agar

[所属分类:培养基配方] [发布时间:2021-11-30] [发布人:网站管理员2] [阅读次数:] [返回]

MacConkey Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of gelatin...............................17.0g

Agar ..................................................................13.5g

Lactose..............................................................10.0g

NaCl....................................................................5.0g

Bile salts..............................................................1.5g

Pancreatic digest of casein..................................1.5g

Peptic digest of animal tissue..............................1.5g

Neutral Red.......................................................0.03g

Crystal Violet ...................................................1.0mg

pH 7.1 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat while stirring until boil

ing. Autoclave for 15 min at 15 psi pressure–121°C.

Pour into sterile Petri dishes or distribute into sterile

tubes.

Use: For the selective isolation, cultivation, and dif

ferentiation of coliforms and enteric pathogens based

on the ability to ferment lactose. Lactose-fermenting

organisms appear as red to pink colonies. Lactose

nonfermenting organisms appear as colorless or

transparent colonies.