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M9 Medium

[所属分类:培养基配方] [发布时间:2021-11-30] [发布人:网站管理员2] [阅读次数:] [返回]

M9 Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Na2HPO4 .............................................................6.0g

KH2PO4...............................................................3.0g

NH4Cl .................................................................1.0g

NaCl....................................................................0.5g

Glucose solution ........................................... 10.0mL

MgSO4·7H2O solution .................................... 1.0mL

Thiamine·HCl solution.................................... 1.0mL

CaCl2 solution................................................. 1.0mL

pH 7.0 ± 0.2 at 25°C

Glucose Solution:

Composition per 100.0mL:

D-Glucose ..........................................................20.0g

Preparation of Glucose Solution: Add glucose

to distilled/deionized water and bring volume to

1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi

pressure–121°C.

MgSO4·7H2O Solution:

Composition per liter:

MgSO4·7H2O ..................................................246.5g

Preparation of MgSO4·7H2O Solution: Add

MgSO4·7H2O to distilled/deionized water and bring

volume to 1.0L. Mix thoroughly. Autoclave for 15

min at 15 psi pressure–121°C.

Thiamine·HCl Solution:

Composition per 10.0mL:

Thiamine·HCl ................................................10.0mg

Preparation of Thiamine·HCl Solution: Add

thiamine·HCl to distilled/deionized water and bring

volume to 1.0L. Mix thoroughly. Filter sterilize.

CaCl2 Solution:

Composition per liter:

CaCl2 solution...................................................14.7g

Preparation of CaCl2 Solution: Add CaCl2 so

lution to distilled/deionized water and bring volume

to 1.0L. Mix thoroughly. Autoclave for 15 min at 15

psi pressure–121°C.

Preparation of Medium: Add components, ex

cept MgSO4·7H2O solution, glucose solution, thia

mine·HCl solution, and CaCl2 solution, to distilled/

deionized water and bring volume to 987.0mL. Mix

thoroughly. Adjust pH to 7.0. Autoclave for 15 min

at 15 psi pressure–121°C. Cool to room temperature.

Aseptically add sterile MgSO4·7H2O solution, sterile

glucose solution, sterile thiamine·HCl solution, and

sterile CaCl2 solution. Mix thoroughly. Distribute

into tubes or flasks.

Use: For the cultivation and maintenance of Escher

ichia coli and a variety of other bacteria.

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M9 Medium