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LPM Agar with Esculin and Ferric Iron (BAM M82)

[所属分类:培养基配方] [发布时间:2021-11-29] [发布人:网站管理员2] [阅读次数:] [返回]

LPM Agar with

Esculin and Ferric Iron

(BAM M82)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................15.0g

Glycine anhydride.............................................10.0g

LiCl .....................................................................5.0g

NaCl....................................................................5.0g

Pancreatic digest of casein..................................5.0g

Peptic digest of animal tissue..............................5.0g

Beef extract.........................................................3.0g

Phenylethyl alcohol.............................................2.5g

Esculin ................................................................1.0g

Ferric ammonium citrate.....................................0.5g

Moxalactam solution....................................... 2.0mL

pH 7.3 ± 0.2 at 25°C

Moxalactam Solution:

Composition per 10.0mL:

Moxalactam ........................................................0.1g

Preparation of Moxalactam Solution: Add

moxalactam to distilled/deionized water and bring

volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept moxalactam solution, to distilled/deionized wa

ter and bring volume to 998.0mL. Mix thoroughly.

Gently heat while stirring and bring to boiling. Auto

clave for 12 min at 15 psi pressure–121°C. Cool to

45°–50°C. Aseptically add 2.0mL of sterile moxalac

tam solution. Mix thoroughly. Pour into sterile Petri

dishes or distribute into sterile tubes.

Use: For the isolation and cultivation of Listeria

monocytogenes.