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Lowenstein-Jensen Medium without Glycerol

[所属分类:培养基配方] [发布时间:2021-11-29] [发布人:网站管理员2] [阅读次数:] [返回]

Lowenstein-Jensen Medium

without Glycerol

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Potato starch......................................................30.0g

Asparagine ..........................................................3.6g

KH2PO4...............................................................2.4g

Magnesium citrate...............................................0.6g

Malachite green...................................................0.4g

MgSO4·7H2O ....................................................0.24g

Homogenized whole egg .................................. 1.0L

Homogenized Whole Egg:

Composition per liter:

Whole eggs ......................................................18–24

Preparation of Homogenized Whole Egg:

Use fresh eggs, less than 1 week old. Scrub the shells

with soap. Let stand in a soap solution for 30 min.

Rinse in running water. Soak eggs in 70% ethanol for

15 min. Break the eggs into a sterile container. Ho

mogenize by shaking. Filter through four layers of

sterile cheesecloth into a sterile graduated cylinder.

Measure out 1.0L.

Preparation of Medium: Add components, ex

cept fresh egg mixture, to 600.0mL of distilled/

deionized water. Mix thoroughly. Gently heat while

stirring and bring to boiling. Autoclave for 15 min at

15 psi pressure–121°C. Cool to 50°C. Aseptically

add 1.0L of homogenized whole egg. Mix thorough

ly. Distribute into sterile screw-capped tubes. Place

tubes in a slanted position. Inspissate at 85°C (moist

heat) for 45 min.

Use: For the cultivation and maintenance of Myco

bacterium species, especially Mycobacterium bovis

and other species that are sensitive to glycerol.