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Liver Infusion Sake Medium

[所属分类:培养基配方] [发布时间:2021-11-29] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Liver, fresh......................................................400.0g

Agar ..................................................................15.0g

Sake............................................................. 400.0mL

Preparation of Medium: Finely mince liver and

add to 600.0mL of distilled/deionized water. Gently

heat and bring to boiling. Continue boiling for 5–10

min. Filter through Whatman #2 filter paper. To fil

trate, add agar and sake. Bring volume to 1.0L with

distilled/deionized water. Mix thoroughly. Gently

heat and bring to boiling. Distribute into tubes or

flasks. Autoclave for 25 min at 15 psi pressure–

121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of Lacto

bacillus fructivorans and Lactobacillus homohiochi.