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Liver Broth

[所属分类:培养基配方] [发布时间:2021-11-29] [发布人:网站管理员2] [阅读次数:] [返回]

Liver Broth

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Beef liver, fresh...............................................453.0g

Pancreatic digest of casein................................10.0g

K2HPO4...............................................................1.0g

Soluble starch......................................................1.0g

pH 7.6 ± 0.2 at 25°C

Preparation of Medium: Remove the fat from

fresh beef liver. Grind the liver. Add 1.0L of distilled/

deionized water. Gently heat and bring to boiling.

Continue boiling for 60 min. Adjust pH to 7.6. Filter

through cheesecloth. Reserve meat. To filtrate, add

pancreatic digest of casein, K2HPO4, and soluble

starch. Bring volume to 1.0L with distilled/deionized

water. Refilter solution. Add meat particles to test

tubes to a depth of approximately 2cm. Distribute

broth into tubes with meat particles in 15.0mL vol

umes. Autoclave for 20 min at 15 psi pressure–121°C.

Use: For the isolation and cultivation of anaerobic

microorganisms, especially Clostridium botulinum,

from foods.