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Listeria Enrichment Broth, FDA (LEB, FDA)

[所属分类:培养基配方] [发布时间:2021-11-26] [发布人:网站管理员2] [阅读次数:] [返回]

Listeria Enrichment Broth, FDA

(LEB, FDA)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Soybean casein digest broth yeast extract ......... 1.0L

Nalidixic acid solution.................................... 8.0mL

Cycloheximide solution.................................. 5.1mL

Acriflavin·HCl solution .................................. 3.0mL

pH 7.3 ± 0.2 at 25°C

Soybean Casein Digest Broth Yeast Extract:

Composition per liter:

Pancreatic digest of casein................................17.0g

Yeast extract........................................................6.0g

NaCl....................................................................5.0g

Papaic digest of soybean meal............................3.0g

K2HPO4...............................................................2.5g

Glucose ...............................................................2.5g

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Soybean Casein Digest Broth

Yeast Extract: Add components to distilled/deion

ized water and bring volume to 1.0L. Mix thorough

ly. Autoclave for 15 min at 15 psi pressure–121°C.

Nalidixic Acid Solution:

Composition per 100.0mL:

Nalidixic acid......................................................0.5g

Preparation of Nalidixic Acid Solution: Add

nalidixic acid to distilled/deionized water and bring

volume to 100.0mL. Mix thoroughly. Filter sterilize.

Cycloheximide Solution:

Composition per 100.0mL:

Cycloheximide....................................................1.5g

Ethanol.......................................................... 40.0mL

Preparation of Cycloheximide Solution: Add

cycloheximide to 40.0mL of ethanol. Mix thorough

ly. Bring volume to 100.0mL with distilled/deionized

water. Filter sterilize.

Acriflavin·HCl Solution:

Composition per 100.0mL:

Acriflavin·HCl solution ......................................0.5g

Preparation of Acriflavin·HCl Solution: Add

acriflavin·HCl solution to distilled/deionized water

and bring volume to 100.0mL. Mix thoroughly. Filter

sterilize.

Preparation of Medium: Add components—ex

cept nalidixic acid solution, acriflavin solution, and

cycloheximide solution—to distilled/deionized wa

ter and bring volume to 990.0mL. Mix thoroughly.

Gently heat and bring to boiling. Autoclave for 15

min at 15 psi pressure–121°C. Cool to 45°–50°C.

Aseptically add 8.0mL of sterile nalidixic acid solu

tion, 5.1mL of sterile cycloheximide solution, and

3.0mL of sterile acriflavin solution. Mix thoroughly.

Pour into sterile Petri dishes or distribute into sterile

tubes.

Use: For the isolation and enrichment of Listeria

monocytogenes from foods.

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Listeria Enrichment Broth, FDA (LEB, FDA)