培养基配方

您现在的位置：网站首页 >> 培养基配方

LICNR Broth (Lysine Iron Cystine Neutral Red Broth)

[所属分类:培养基配方] [发布时间:2021-11-26] [发布人:网站管理员2] [阅读次数:] [返回]

LICNR Broth

(Lysine Iron Cystine Neutral Red Broth)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 500.0mL:

L-Lysine·HCl .....................................................10.0g

Mannitol..............................................................5.0g

Pancreatic digest of casein..................................5.0g

Yeast extract........................................................3.0g

Glucose ...............................................................1.0g

Salicin .................................................................1.0g

Ferric ammonium citrate.....................................0.5g

L-Cystine .............................................................0.1g

Na2S2O3 ..............................................................0.1g

Neutral Red.....................................................0.025g

Novobiocin solution...................................... 10.0mL

pH 6.2 ± 0.2 at 25°C

Novobiocin Solution:

Composition per 10.0mL:

Novobiocin......................................................0.015g

Preparation of Novobiocin Solution: Add no

vobiocin to distilled/deionized water and bring vol

ume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept novobiocin solution, to distilled/deionized water

and bring volume to 990.0mL. Mix thoroughly. Gen

tly heat and bring to boiling. Continue boiling for 2–

3 min. Distribute into tubes in 10.0mL volumes. Au

toclave for 15 min at 15 psi pressure–121°C. Cool to

45°–50°C. Aseptically add 0.1mL of sterile novobio

cin solution to each tube. Mix thoroughly.

Use: For the rapid, presumptive detection of Salmo

nella in foods, food ingredients, and feed materials.