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Lee’s Agar

[所属分类:培养基配方] [发布时间:2021-11-26] [发布人:网站管理员2] [阅读次数:] [返回]

Lee’s Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................18.0g

Pancreatic digest of casein................................10.0g

Yeast extract......................................................10.0g

Lactose................................................................5.0g

Sucrose................................................................5.0g

CaCO3 .................................................................3.0g

K2HPO4...............................................................0.5g

Bromcresol Purple (0.2% solution) .............. 10.0mL

pH 7.0 ± 0.2 at 25°C

Bromcresol Purple Solution:

Composition per 10.0mL:

Bromcresol Purple ............................................0.02g

Preparation of Bromcresol Purple Solution:

Add Bromcresol Purple to distilled/deionized water

and bring volume to 10.0mL. Mix thoroughly. Filter

sterilize.

Preparation of Medium: Add components, ex

cept Bromcresol Purple solution, to distilled/deion

ized water and bring volume to 990.0mL. Mix

thoroughly. Adjust pH to 7.0. Gently heat and bring

to boiling. Autoclave for 20 min at 15 psi pressure–

121°C. Cool to 45°–50°C. Aseptically add sterile

Bromcresol Purple solution. Mix thoroughly. Pour

into sterile, chilled Petri dishes in 20–25mL vol

umes. Swirl flask while dispensing to evenly sus

pend CaCO3. Dry plates at 30°C for 18–24 hr before

use.

Use: For the isolation, cultivation, and enumeration

of Lactobacillus bulgaricus from yogurt.