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Lead Acetate Agar

[所属分类:培养基配方] [发布时间:2021-11-26] [发布人:网站管理员2] [阅读次数:] [返回]

Lead Acetate Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................15.0g

Peptone .............................................................15.0g

Proteose peptone.................................................5.0g

Glucose ...............................................................1.0g

Lead acetate ........................................................0.2g

Na2S2O3 ............................................................0.08g

pH 6.6 ± 0.2 at 25°C

Preparation of Medium: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix

thoroughly. Gently heat and bring to boiling. Distrib

ute into tubes or flasks. Autoclave for 15 min at 15 psi

pressure–121°C. Pour into sterile Petri dishes or leave

in tubes. Allow tubes to cool in a slanted position.

Use: For the cultivation and differentiation of Gram

negative coliform bacteria based on H2S production.

Bacteria that produce H2S turn the medium brown.