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LB Medium for Χ1776

[所属分类:培养基配方] [发布时间:2021-11-26] [发布人:网站管理员2] [阅读次数:] [返回]

LB Medium for Χ1776

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl..................................................................10.0g

Pancreatic digest of casein................................10.0g

Yeast extract........................................................5.0g

Glucose solution ........................................... 10.0mL

Diaminopimelic acid solution....................... 10.0mL

Thymidine solution....................................... 10.0mL

pH 7.0 ± 0.2 at 25°C

Glucose Solution:

Composition per 10.0mL:

D-Glucose ............................................................0.8g

Preparation of Glucose Solution: Add D-glu

cose to distilled/deionized water and bring volume to

10.0mL. Mix thoroughly. Filter sterilize.

Diaminopimelic Acid Solution:

Composition per 10.0mL:

DL-Diaminopimelic acid .....................................0.1g

Preparation of Diaminopimelic Acid Solu

tion: Add diaminopimelic acid to distilled/deionized

water and bring volume to 10.0mL. Mix thoroughly.

Filter sterilize.

Thymidine Solution:

Composition per 10.0mL:

Thymidine.........................................................0.02g

Preparation of Thymidine Solution: Add thy

midine to distilled/deionized water and bring volume

to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components—ex

cept glucose solution, diaminopimelic acid solution,

and thymidine solution—to distilled/deionized water

and bring volume to 970.0mL. Mix thoroughly. Gen

tly heat and bring to boiling. Autoclave for 15 min at

15 psi pressure–121°C. Cool to 45°–50°C. Aseptical

ly add sterile glucose solution, diaminopimelic acid

solution, and thymidine solution. Mix thoroughly.

Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation and maintenance of Bacillus

subtilis and Escherichia coli.