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Lauryl Tryptose Mannitol Broth with Tryptophan

[所属分类:培养基配方] [发布时间:2021-11-26] [发布人:网站管理员2] [阅读次数:] [返回]

Lauryl Tryptose Mannitol

Broth with Tryptophan

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein................................20.0g

Lactose................................................................5.0g

NaCl....................................................................5.0g

K2HPO4.............................................................2.75g

KH2PO4.............................................................2.75g

Sodium lauryl sulfate..........................................0.1g

L-Tryptophan.......................................................0.2g

pH 6.8 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from Oxoid.

Preparation of Medium: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix

thoroughly. Distribute into tubes containing an inverted

Durham tube in 10.0mL volumes. Autoclave for 10 min

at 10 psi pressure–115°C. Cool broth quickly to 25°C.

Use: For the detection of Escherichia coli.