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Lactose Gelatin Medium (BAM M75)

[所属分类:培养基配方] [发布时间:2021-11-25] [发布人:网站管理员2] [阅读次数:] [返回]

Lactose Gelatin Medium

(BAM M75)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Gelatin.............................................................120.0g

Tryptose ............................................................15.0g

Lactose..............................................................10.0g

Yeast extract......................................................10.0g

Phenol Red (1.0% in ethanol solution) ........... 5.0mL

pH 7.5 ± 0.2 at 25°C

Preparation of Medium: Add tryptose, yeast ex

tract, and lactose to distilled/deionized water and

bring volume to 400.0mL. Mix thoroughly. Add gel

atin to distilled/deionized water and bring volume to

590.0mL Gently heat gelatin solution while stirring

and bring to 50°–60°C. Add Phenol Red. Mix the two

solutions together. Distribute into tubes in 10.0mL

volumes. Autoclave for 10 min at 15 psi pressure–

121°C. If medium is not used in 8 hr, deoxygenate by

heating to 50°–70°C for 2–3 hr prior to inoculation.

Use: For the cultivation of Clostridium perfringens.