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Lactobacillus Orotic Acid Medium

[所属分类:培养基配方] [发布时间:2021-11-25] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Milk, peptonized...............................................15.0g

Glucose .............................................................10.0g

Yeast extract........................................................5.0g

KH2PO4...............................................................2.0g

Sorbitan monooleate complex ............................1.0g

Orotic acid .....................................................25.0mg

D-Pantothenic acid ...........................................0.2mg

Tomato juice ............................................... 100.0mL

L-Cysteine·HCl·H2O solution ......................... 7.5mL

pH 6.8 ± 0.2 at 25°C

L-Cysteine·HCl·H2O Solution:

Composition per 10.0mL:

L-Cysteine·HCl·H2O .........................................0.15g

Preparation of L-Cysteine·HCl·H2O Solu

tion: Add L-cysteine·HCl·H2O to distilled/deionized

water and bring volume to 10.0mL. Mix thoroughly.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Continue boiling for 2–3 min. Distribute into bottles

in 20.0mL volumes. Add 0.15mL of L-cys

teine·HCl·H2O solution to each bottle containing

20.0mL of medium. Autoclave for 15 min at 15 psi

pressure–121°C. Screw caps tightly to maintain re

duced conditions.

Use: For the cultivation of Lactobacillus helveticus.