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Lactobacilli Broth, AOAC (Lactobacilli Broth, Association of Official Analytical Chemists)

[所属分类:培养基配方] [发布时间:2021-11-25] [发布人:网站管理员2] [阅读次数:] [返回]

Lactobacilli Broth, AOAC

(Lactobacilli Broth, Association of

Official Analytical Chemists)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Milk, peptonized...............................................15.0g

Glucose .............................................................10.0g

Yeast extract........................................................5.0g

KH2PO4...............................................................2.0g

Sorbitan monooleate complex ............................1.0g

Tomato juice ............................................... 100.0mL

pH 6.8 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Continue boiling for 2–3 min. Distribute into tubes in

10.0mL volumes. Autoclave for 15 min at 15 psi

pressure–121°C.

Use: For the cultivation and preparation of inocula

of stock cultures of Lactobacillus casei ATCC 7469,

Lactobacillus fermentum ATCC 9338, Lactobacillus

leichmannii ATCC 4797, and Lactobacillus viride

scens ATCC 12706 used in the microbiological assay

of vitamins.