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Lactic Streak Agar

[所属分类:培养基配方] [发布时间:2021-11-25] [发布人:网站管理员2] [阅读次数:] [返回]

Lactic Streak Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................15.0g

Sodium carboxymethylcellulose.......................10.0g

Calcium citrate..................................................10.0g

Beef extract.........................................................5.0g

Papaic digest of soybean meal............................5.0g

Polypeptone ........................................................5.0g

Yeast extract........................................................5.0g

Lactose................................................................1.5g

L-Arginine·HCl....................................................1.5g

Bromcresol Purple (0.1% solution) .............. 20.0mL

pH 6.0 ± 0.1 at 25°C

Preparation of Medium: Add components—ex

cept Bromcresol Purple solution, calcium citrate, and

sodium carboxymethylcellulose—to distilled/deion

ized water and bring volume to 800.0mL. Mix thor

oughly. Gently heat and bring to boiling. In a blender,

add the calcium citrate and sodium carboxymethyl

cellulose to 200.0mL of distilled/deionized water.

Blend until mixed. Add the 200.0mL of citrate/car

boxymethylcellulose solution to the hot agar solu

tion. Mix thoroughly. Adjust pH to 6.0. Distribute

into flasks in 100.0mL volumes. Autoclave for 10

min at 10 psi pressure–115°C. Cool to 45°–50°C.

Aseptically add 2.0mL of sterile Bromcresol Purple

to each flask. Mix thoroughly. Pour into sterile Petri

dishes. Allow plates to dry at 37°C for 1 hr before

use.

Use: For the differentiation of lactic streptococci.

Bacteria that produce acid from lactose appear as yel

low colonies.