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Lactic Acid Bacteria Broth

[所属分类:培养基配方] [发布时间:2021-11-25] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Sodium acetate..................................................12.0g

Pancreatic digest of casein................................10.0g

Yeast autolysate ..................................................5.0g

Glucose .............................................................10.0g

Solution A....................................................... 5.0mL

Solution B ....................................................... 5.0mL

pH 5.1 ± 0.2 at 25°C

Source: Yeast autolysate is available from Oxoid.

Solution A:

Composition per 100.0mL:

K2HPO4.............................................................10.0g

KH2PO4.............................................................10.0g

Preparation of Solution A: Add components to

distilled/deionized water and bring volume to

100.0mL. Mix thoroughly.

Solution B:

Composition per 100.0mL:

MgSO4·7H2O......................................................4.0g

FeSO4·7H2O........................................................0.2g

MnSO4·H2O........................................................0.2g

NaCl....................................................................0.2g

Preparation of Solution B: Add components to

distilled/deionized water and bring volume to

100.0mL. Mix thoroughly.

Preparation of Medium: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix

thoroughly. Distribute into tubes or flasks. Autoclave

for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Lactobacillus malefer

mentans.