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L15 Medium, Modified Leibovitz (BAM M73)

[所属分类:培养基配方] [发布时间:2021-11-25] [发布人:网站管理员2] [阅读次数:] [返回]

L15 Medium, Modified Leibovitz

(BAM M73)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl....................................................................8.0g

DL-Threonine.......................................................0.6g

Sodium pyruvate.................................................0.6g

DL-Alanine...........................................................0.5g

L-Arginine, free base ...........................................0.5g

KCl......................................................................0.4g

L-Asparagine·H2O ...............................................0.3g

L-Histidine, free base...........................................0.3g

L-Glutamine.........................................................0.3g

L-Isoleucine .........................................................0.3g

L-Phenylalanine...................................................0.3g

L-Tyrosine............................................................0.3g

DL-Methionine.....................................................0.2g

DL-Valine .............................................................0.2g

Glycine................................................................0.2g

L-Serine ...............................................................0.2g

Na2HPO4, anhydrous ..........................................0.2g

CaCl2, anhydrous ................................................0.1g

L-Cysteine, free base ...........................................0.1g

L-Leucine·HCl.....................................................0.1g

MgCl2, anhydrous ...........................................0.094g

D-Galactose .......................................................0.09g

KH2PO4.............................................................0.06g

L-Tryptophan.....................................................0.02g

Phenol Red........................................................0.01g

i-Inositol...........................................................2.0mg

Choline chloride...............................................1.0mg

D-Calcium pantothenate ...................................1.0mg

Folic acid..........................................................1.0mg

Nicotinamide....................................................1.0mg

Pyridoxine·HCl ................................................1.0mg

Thiamine monophosphate·2H2O......................1.0mg

Riboflavin-5-phosphate ...................................0.1mg

pH 7.5 ± 0.2 at 25°C

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Filter sterilize. Store at 5°C.

Use: For the cultivation of oysters used for the

growth of enteroviruses.

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L15 Medium, Modified Leibovitz (BAM M73)