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King’s Medium A

[所属分类:培养基配方] [发布时间:2021-11-24] [发布人:网站管理员2] [阅读次数:] [返回]

King’s Medium A

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Proteose peptone...............................................20.0g

Agar ..................................................................15.0g

Glycerol ............................................................10.0g

K2SO4................................................................10.0g

MgCl2·6H2O .......................................................3.5g

pH 7.2–7.4 ± 0.2 at 25°C

Preparation of Medium: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix

thoroughly. Gently heat and bring to boiling. Distrib

ute into tubes or flasks. Autoclave for 15 min at 15 psi

pressure–121°C. Pour into sterile Petri dishes or leave

in tubes.

Use: For the nonselective isolation, cultivation, and

pigment production of Pseudomonas.