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Kim-Goepfert Agar (KG Agar)

[所属分类:培养基配方] [发布时间:2021-11-24] [发布人:网站管理员2] [阅读次数:] [返回]

Kim-Goepfert Agar

(KG Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1001.0mL:

Solution A................................................... 900.0mL

Egg yolk emulsion, 50%............................. 100.0mL

Polymyxin B solution ..................................... 1.0mL

pH 6.8 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Solution A:

Composition per 900.0mL:

Agar ..................................................................18.0g

Peptone ...............................................................1.0g

Yeast extract........................................................0.5g

Phenol Red......................................................0.025g

Preparation of Solution A: Add components to

distilled/deionized water and bring volume to

900.0mL. Mix thoroughly. Adjust pH to 6.8. Auto

clave for 15 min at 15 psi pressure–121°C. Cool to

45°–50°C.

Egg Yolk Emulsion, 50%:

Composition per 100.0mL:

Chicken egg yolks..................................................11

Whole chicken egg ..................................................1

NaCl (0.9% solution).................................... 50.0mL

Preparation of Egg Yolk Emulsion, 50%:

Soak eggs with 1:100 dilution of saturated mercuric

chloride solution for 1 min. Crack eggs and separate

yolks from whites. Mix egg yolks with 1 chicken egg.

Measure 50.0mL of egg yolk emulsion and add to

50.0mL of 0.9% NaCl solution. Mix thoroughly. Filter

sterilize. Warm to 45°–50°C.

Polymyxin B Solution:

Composition per 5.0mL:

Polymyxin B sulfate...................................500,000U

Preparation of Polymyxin B Solution: Add

polymyxin B to distilled/deionized water and bring

volume to 5.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: To 900.0mL of cooled,

sterile solution A, aseptically add 100.0mL of sterile

egg yolk emulsion, 50%, and 1.0mL of sterile poly

myxin B solution. Mix thoroughly. Pour into sterile

Petri dishes. Allow plates to dry in the dark at 30°C for

24 hr before using.

Use: For the cultivation and differentiation of Bacil

lus cereus.

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Kim-Goepfert Agar (KG Agar)