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KC Bottom Agar

[所属分类:培养基配方] [发布时间:2021-11-24] [发布人:网站管理员2] [阅读次数:] [返回]

KC Bottom Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................10.0g

Pancreatic digest of casein................................10.0g

KCl......................................................................2.5g

NaCl....................................................................2.5g

CaCl2 solution................................................. 1.0mL

CaCl2 Solution:

Composition per 10.0mL:

CaCl2·2H2O ......................................................1.47g

Preparation of CaCl2 Solution: Add 1.47g of

CaCl2·2H2O to distilled/deionized water and bring

volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept CaCl2 solution, to distilled/deionized water and

bring volume to 999.0mL. Mix thoroughly. Gently

heat and bring to boiling. Autoclave for 15 min at 15

psi pressure–121°C. Cool to 45°–50°C. Aseptically

add 1.0mL of CaCl2 solution. Mix thoroughly. Pour

into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation and maintenance of Escher

ichia coli.