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Irgasan Ticarcillin Chlorate Broth (ITC Broth) (BAM M67)

[所属分类:培养基配方] [发布时间:2021-11-24] [发布人:网站管理员2] [阅读次数:] [返回]

Irgasan Ticarcillin Chlorate Broth

(ITC Broth)

(BAM M67)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

MgCl2·6H20 ......................................................60.0g

Pancreatic digest of casein................................10.0g

NaCl....................................................................5.0g

KClO4 .................................................................1.0g

Yeast extract........................................................1.0g

Malachite Green (0.2% solution).................... 5.0mL

Irgasan solution............................................... 1.0mL

Ticarcillin solution.......................................... 1.0mL

pH 7.6 ± 0.2 at 25°C

Irgasan Solution:

Composition per 10.0mL:

Irgasan (triclosan) ............................................1.0mg

Preparation of Irgasan Solution: Add Irgasan

to distilled/deionized water and bring volume to

10.0mL. Mix thoroughly. Filter sterilize.

Ticarcillin Solution:

Composition per 10.0mL:

Ticarcillin.........................................................1.0mg

Preparation of Ticarcillin Solution: Add ticar

cillin to distilled/deionized water and bring volume

to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept Irgasan solution and ticarcillin solution, to dis

tilled/deionized water and bring volume to 998.0mL.

Mix thoroughly. Autoclave for 15 min at 15 psi pres

sure–121°C. Cool to 45°–50°C. Adjust to pH 7.6.

Aseptically add 1.0mL of Irgasan solution and 1.0mL

of ticarcillin solution. Mix thoroughly. Aseptically

distribute into sterile tubes or flasks.

Use: For the selective isolation and cultivation of

Yersinia species.