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Infection Medium (IM)

[所属分类:培养基配方] [发布时间:2021-11-23] [发布人:网站管理员2] [阅读次数:] [返回]

Infection Medium

(IM)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 100.0mL:

Pancreatic digest of gelatin...............................0.05g

Bile salts No. 3..................................................0.05g

Brain heart, solids from infusion ......................0.02g

Peptic digest of animal tissue ...........................0.02g

NaCl................................................................0.017g

Glucose .............................................................0.01g

Na2HPO4..........................................................8.0mg

Earle’s balanced salts solution...................... 80.0mL

Fetal bovine serum, heat inactivated

(2 hr at 55°C).......................................... 20.0mL

pH 7.4 ± 0.2 at 25°C

Earle’s Balanced Salts Solution:

Composition per liter:

NaCl....................................................................6.8g

NaHCO3..............................................................2.2g

Glucose ...............................................................1.0g

KCl......................................................................0.4g

CaCl2·2H2O ....................................................0.265g

MgSO4·7H2O......................................................0.2g

NaH2PO4·H2O...................................................0.14g

Preparation of Earle’s Balanced Salts Solu

tion: Add components to distilled/deionized water

and bring volume to 1.0L. Mix thoroughly. Filter

sterilize.

Preparation of Medium: Combine components.

Mix thoroughly. Filter sterilize. Store at 4°–10°C.

Use: For the screening of Escherichia coli for patho

genicity using the HeLa cell test for invasiveness.