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培养基配方

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[所属分类:培养基配方] [发布时间:2021-11-23] [发布人:网站管理员2] [阅读次数:] [返回]

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山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1011.0mL:

Agar ..................................................................15.0g

Peptone................................................................5.0g

Yeast extract........................................................1.0g

Basal salts solution............................................. 1.0L

Lactose solution ................................................10.0g

Trace elements solution .................................. 1.0mL

Basal Salts Solution:

Composition per liter:

MgSO4 ................................................................0.5g

Phosphate solution ........................................ 20.0mL

(NH4)SO4 (36% solution) ............................... 5.0mL

Phosphate Solution:

Composition per liter:

K2HPO4.............................................................95.0g

NaH2PO4·2H2O.................................................78.0g

Preparation of Phosphate Solution: Add compo

nents to distilled/deionized water and bring volume

to 1.0L. Mix thoroughly.

Preparation of Basal Salts Medium: Add com

ponents to distilled/deionized water and bring vol

ume to 1.0L. Mix thoroughly. Adjust pH to 6.8-7.0.

Lactose Solution:

Composition per 10.0mL:

Lactose................................................................2.5g

Preparation of Lactose Solution: Add lactose

to distilled/deionized water and bring volume to

10.0mL. Mix thoroughly. Filter sterilize. Warm to

50°–55°C.

Trace Elements Solution:

Composition per liter:

Disodium EDTA .................................................0.5g

FeSO4·7H2O........................................................0.2g

H3BO3 ...............................................................0.03g

CoCl2·6H2O ......................................................0.02g

ZnSO4·7H2O .....................................................0.01g

MnCl2·4H2O ....................................................3.0mg

Na2MoO4·2H2O ...............................................3.0mg

NiCl2·6H2O......................................................2.0mg

CaCl2·2H2O .....................................................1.0mg

Preparation of Trace Elements Solution: Add

components to distilled/deionized water and bring

volume to 1.0L. Mix thoroughly. Autoclave for 15

min at 15 psi pressure–121°C.

Preparation of Medium: Combine components,

except lactose solution and trace elements solution.

Mix thoroughly. Gently heat and bring to boiling.

Autoclave for 15 min at 15 psi pressure–121°C. Cool

to 50°–55°C. Aseptically add 10.0mL of sterile lac

tose solution and 1.0mL of sterile trace elements so

lution. Mix thoroughly. Pour into sterile Petri dishes

or distribute into sterile tubes.

Use: For the cultivation of Bacillus species.

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