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HYA Agar

[所属分类:培养基配方] [发布时间:2021-11-23] [发布人:网站管理员2] [阅读次数:] [返回]

HYA Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................15.0g

Proteose peptone No. 3.....................................10.0g

Beef extract.........................................................1.0g

Lactose solution............................................ 10.0mL

Galactose solution......................................... 10.0mL

Glucose solution ........................................... 10.0mL

pH 6.8 ± 0.2 at 25°C

Lactose Solution:

Composition per 10.0mL:

Lactose................................................................5.0g

Preparation of Lactose Solution: Add lactose

to distilled/deionized water and bring volume to

10.0mL. Mix thoroughly. Filter sterilize.

Galactose Solution:

Composition per 10.0mL:

Galactose.............................................................2.5g

Preparation of Galactose Solution: Add galac

tose to distilled/deionized water and bring volume to

10.0mL. Mix thoroughly. Filter sterilize.

Glucose Solution:

Composition per 10.0mL:

Glucose ...............................................................2.5g

Preparation of Glucose Solution: Add glucose

to distilled/deionized water and bring volume to

10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components—ex

cept lactose solution, galactose solution, and glucose

solution—to distilled/deionized water and bring vol

ume to 970.0mL. Mix thoroughly. Gently heat and

bring to boiling. Adjust pH to 6.8. Autoclave for 15

min at 15 psi pressure–121°C. Cool to 45°–50°C.

Aseptically add sterile lactose solution, galactose so

lution, and glucose solution. Mix thoroughly. Pour

into sterile Petri dishes.

Use: For the cultivation of acidogenic microorgan

isms, especially Lactobacillus bulgaricus and Strep

tococcus thermophilus, from foods.