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Horie Arabinose Ethyl Violet Broth (HAEB)

[所属分类:培养基配方] [发布时间:2021-11-23] [发布人:网站管理员2] [阅读次数:] [返回]

Horie Arabinose Ethyl Violet Broth

(HAEB)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl..................................................................30.0g

Peptone................................................................5.0g

Beef extract.........................................................3.0g

Bromthymol Blue .............................................0.03g

Ethyl Violet......................................................1.0mg

Arabinose solution ...................................... 100.0mL

pH 9.0 ± 0.2 at 25°C

Arabinose Solution:

Composition per 100.0mL:

Arabinose............................................................5.0g

Preparation of Arabinose Solution: Add arabi

nose to distilled/deionized water and bring volume to

100.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept arabinose solution, to distilled/deionized water

and bring volume to 900.0mL. Mix thoroughly. Gen

tly heat and bring to boiling. Adjust pH to 9.0. Auto

clave for 15 min at 15 psi pressure–121°C. Cool to

45°–50°C. Aseptically add sterile arabinose solution.

Mix thoroughly. Aseptically distribute into sterile

tubes or flasks.

Preparation of Medium: Dissolve all ingredi

ents, except arabinose, in 900.0mL of distilled water.

Adjust the pH to 9.0 ± 0.2 and sterilize at 121°C for

15 min. Cool and aseptically add 100.0mL of a filter

sterilized 5% arabinose solution in distilled water.

After mixing, aseptically dispense the medium into

sterile tubes or bottles.

Use: For the cultivation of Vibrio species from

foods.