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HiCrome Salmonella Agar (Salmonella Agar, HiCrome)

[所属分类:培养基配方] [发布时间:2021-11-23] [发布人:网站管理员2] [阅读次数:] [返回]

HiCrome Salmonella Agar

(Salmonella Agar, HiCrome)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................13.0g

Peptic digest of animal tissue..............................6.0g

Chromogenic mixture .........................................5.4g

Yeast extract........................................................2.5g

Bile salt mixture..................................................1.0g

pH 7.7 ± 0.2 at 25°C

Source: This medium is available from Fluka, Sig

ma-Aldrich.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat while stirring and bring

to boiling. Mix to completely dissolve components.

Do not autoclave. Cool to 50°C. Pour into sterile Pet

ri dishes.

Use: A selective chromogenic medium used for the

isolation and differentiation of Salmonella species

from coliforms in food and water. E. coli and Salmo

nella are easily distinguishable due to the colony

characteristics. Salmonella give light purple colonies

with a halo. E. coli has a characteristic blue color.

Other organisms give colorless colonies. The charac

teristic light purple and blue color is due to the chro

mogenic mixture.