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HiCrome Enterococci Broth (Enterococci HiCrome Broth)

[所属分类:培养基配方] [发布时间:2021-11-23] [发布人:网站管理员2] [阅读次数:] [返回]

HiCrome Enterococci Broth

(Enterococci HiCrome Broth)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Peptone, special ................................................10.0g

NaCl....................................................................5.0g

Polysorbate 80.....................................................2.0g

NaH2PO4 ...........................................................1.25g

NaN3 ...................................................................0.3g

Chromogenic mixture .......................................0.04g

pH 7.5 ± 0.2 at 25°C

Source: This medium is available from Fluka, Sig

ma-Aldrich.

Caution: Sodium azide is toxic. It also has a tenden

cy to form explosive metal azides with plumbing ma

terials. It is advisable to use enough water to flush off

the disposables.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Distribute into tubes or flasks. Auto

clave for 15 min at 15 psi pressure–121°C.

Use: For the rapid and easy identification and differ

entiation of enterococci. It contains a chromogenic

substrate which aids in the detection of enterococci,

especially in water samples.