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HiCrome ECC Selective Agar (ECC Selective Agar, HiCrome)

[所属分类:培养基配方] [发布时间:2021-11-23] [发布人:网站管理员2] [阅读次数:] [返回]

HiCrome ECC Selective Agar

(ECC Selective Agar, HiCrome)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................10.0g

Peptone, special ..................................................6.0g

Casein enzymic hydrolysate ...............................3.3g

NaCl....................................................................2.0g

Sodium pyruvate.................................................1.0g

Tryptophan..........................................................1.0g

Sorbitol................................................................1.0g

Na2HPO4 .............................................................1.0g

NaH2PO4 .............................................................0.6g

Tergitol 7...........................................................0.15g

Chromogenic mixture .......................................0.43g

Cefulodin .........................................................5.0mg

pH 6.8 ± 0.2 at 25°C

Source: This medium is available from Fluka, Sig

ma-Aldrich.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly and heat with frequent agitation until

components are completely dissolved (approximate

ly 35 min). Do not autoclave. Cool to 45°–50°C.

Pour into sterile Petri dishes. Medium may show haz

iness.

Use: For detection of Escherichia coli and coliforms

in water and food samples using pour plate or streak

plate methods.Tergitol inhibits Gram-positive as well

as some Gram-negative bacteria other than

coliforms. The cefsulodin inhibits Pseudomonas and

Aeromonas species. The chromogenic mixture con

tains two chromogenic substrates as Salmon-GAL

and X-glucuronide. The enzyme β-D-galactosidase

produced by coliforms cleaves Salmon-GAL, result

ing in the salmon to red coloration of coliform colo

nies. The enzyme β-D-glucuronidase produced by E.

coli cleaves X-glucuronide. E. coli forms dark blue to

violet colored colonies due to cleavage of both Salm

on-GAL and X-glucuronide. The addition of tryp

tophan improves the indole reaction.