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HiCrome ECC Agar (ECC Agar, HiCrome)

[所属分类:培养基配方] [发布时间:2021-11-23] [发布人:网站管理员2] [阅读次数:] [返回]

HiCrome ECC Agar

(ECC Agar, HiCrome)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Chromogenic mixture .......................................20.3g

Agar ..................................................................15.0g

Peptone, special ..................................................5.0g

NaCl....................................................................5.0g

Na2HPO4.............................................................3.5g

Yeast extract........................................................3.0g

Lactose................................................................2.5g

KH2PO4...............................................................1.5g

Neutral Red.......................................................0.03g

pH 6.8 ± 0.2 at 25°C

Source: This medium is available from Fluka, Sig

ma-Aldrich.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat while stirring and bring

to boiling. Autoclave for 15 min at 15 psi pressure–

121°C. Pour into sterile Petri dishes.

Use: A differential medium for presumptive identi

fication of E. coli and other coliforms in food sam

ples. The chromogenic mixture contains two

chromogens as X-glucuronide and Salmon-GAL. X

glucuronide is cleaved by the the enzyme β-glucu

ronidase produced by E. coli. Salmon-GAL is

cleaved by the enzyme galactosidase produced by the

majority of coliforms, including E. coli.