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HiCrome™ Aureus Agar Base (Aureus Agar Base) (HiCrome Staphylococcus aureus Agar) (Staphylococcus aureus Agar, HiCrome)

[所属分类:培养基配方] [发布时间:2021-11-22] [发布人:网站管理员2] [阅读次数:] [返回]

HiCrome™ Aureus Agar Base

(Aureus Agar Base)

(HiCrome Staphylococcus aureus Agar)

(Staphylococcus aureus Agar, HiCrome)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................20.0g

Casein enzymic hydrolysate .............................12.0g

Sodium pyruvate...............................................10.0g

Beef extract.........................................................6.0g

LiCl .....................................................................5.0g

Yeast extract........................................................5.0g

Pancreatic digest of of gelatin.............................3.0g

Chromogenic mixture .........................................2.1g

Egg tellurite emulsion................................... 50.0mL

pH 7.4 ± 0.2 at 25°C

Source: This medium is available from Fluka, Sig

ma-Aldrich.

Caution: Lithium chloride is harmful. Avoid bodily

contact and inhalation of vapors. On contact with

skin, wash with plenty of water immediately.

Egg Yolk Tellurite Emulsion:

Composition per 100.0mL:

Sterile saline.................................................. 64.0mL

Egg Yolk ....................................................... 30.0mL

Sterile potassium tellurite solution, 3.5% ....... 6.0mL

Preparation of Medium: Add components, ex

cept egg yolk tellurite emulsion, to distilled/deion

ized water and bring volume to 950.0mL. Mix

thoroughly. Gently heat and bring to boiling. Auto

clave for 15 min at 15 psi pressure–121°C. Cool to

50°C. Aseptically add 50.0mL sterile egg yok tellu

rite emulsion. Mix thoroughly. Pour into sterile Petri

dishes or distribute into sterile tubes.

Use: For the isolation and enumeration of coagulase

positive Staphylococcus aureus. Coagulase positive

S. aureus gives brown black colonies whereas S. epi

dermidis gives yellow, slightly brownish, colonies.