Anthracis Chromogenic Agar

[所属分类:培养基配方] [发布时间:2019-10-21] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Anthracis Chromogenic Agar
Composition per liter:
Proprietary.
Source:  This medium is available as a premixed powder from BIO-
SYNTH International, Inc.
Preparation of Medium: Per manufacturer’s directions.
Use : For the rapid identification and isolation of Bacillus anthracis
based on the detection of phosphatidylcholine-specific phospholipase
C activity by 5-bromo–4-chloro–3-indoxyl-cholinphosphate hydroly-
sis. The medium incorporates chromogenic substrates for detecting
specific enzyme activities in Bacillus anthracis, B. cereus, and B. thu-
ringiensis. The enzymes targeted by the chromogenic medium are not
present in other Bacillus species, allowing for specific isolation of
these three Bacillus species. Inclusion of inhibitory compounds into
the medium prevents the growth of environmental contaminants. The
use of proprietary chromogenic substrates, X-IP and X-CP, allows for
the differentiation of Bacillus anthracis from near-neighbors B. cereus
and B. thuringiensis. Cream to pale teal-blue colored of Bacillus
anthracis after 20–24h, teal-blue colonies of Bacillus anthracis after
36–48 h at 35–37°C. Dark teal-blue colonies of Bacillus cereus/Bacil-
lus thuringiensis after 20–24h at 35–37°C.