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Hektoen Enteric Agar (BAM M61)

[所属分类:培养基配方] [发布时间:2021-11-22] [发布人:网站管理员2] [阅读次数:] [返回]

Hektoen Enteric Agar

(BAM M61)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................14.0g

Lactose..............................................................12.0g

Peptic digest of animal tissue ...........................12.0g

Sucrose..............................................................12.0g

Bile salts..............................................................9.0g

NaCl....................................................................5.0g

Na2S2O3 ..............................................................5.0g

Yeast extract........................................................3.0g

Salicin .................................................................2.0g

Ferric ammonium citrate.....................................1.5g

Acid Fuchsin.......................................................0.1g

Bromthymol Blue ...........................................0.064g

pH 7.5 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems and Oxoid.

Caution: Acid Fuchsin is a potential carcinogen

and care must be taken to avoid inhalation of the

powdered dye and contact with the skin.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat while stirring until com

ponents are dissolved. Do not autoclave. Pour into

sterile Petri dishes. Allow agar to solidify with the

Petri dish covers partially off. Do not store more than

1 day.

Use: For the isolation and cultivation of Gram-neg

ative enteric microorganisms based on lactose or su

crose fermentation and H2S production. For the iso

lation and differentiation of Salmonella and Shigella.

Bacteria that ferment lactose or sucrose appear as

yellow to orange colonies. Bacteria that produce H2S

appear as colonies with black centers.