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Heart Infusion Agar (HIA) (BAM M159)

[所属分类:培养基配方] [发布时间:2021-11-22] [发布人:网站管理员2] [阅读次数:] [返回]

Heart Infusion Agar

(HIA)

(BAM M159)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Proteose Peptone ..............................................15.0g

Agar ..................................................................12.0g

Yeast Extract ...................................................... 5.0g

NaCl....................................................................5.0g

Liver Digest ........................................................2.5g

pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Distribute into tubes or flasks. Autoclave for 15 min

at 15 psi pressure–121°C. Pour into sterile Petri dish

es or leave in tubes.

Use: For the isolation and cultivation of a wide vari

ety of fastidious microorganisms. For the cultivation

and maintenance of Bacillus cereus, Staphylococcus

aureus, and Vibrio vulnificus and Vibrio cholerae. It

can also be used as a base for the preparation of blood

agar in determining hemolytic reactions. When using

for blood agar, reduce volume to 950.0mL to allow

for addition of 50.0mL of defibrinated horse blood.

Blood is added aseptically after autoclaving.