培养基配方

您现在的位置：网站首页 >> 培养基配方

Glutamine Medium

[所属分类:培养基配方] [发布时间:2021-11-22] [发布人:网站管理员2] [阅读次数:] [返回]

Glutamine Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................15.0g

Pancreatic digest of gelatin.................................5.0g

Beef extract.........................................................3.0g

Glutamine/tryptophan solution ..................... 10.0mL

pH 6.8 ± 0.2 at 25°C

Glutamine/Tryptophan Solution:

Composition per liter:

L-Glutamine .................................................100.0mg

L-Tryptophan ...............................................100.0mg

Preparation of Medium: Add components, ex

cept glutamine/tryptophan solution, to distilled/

deionized water and bring volume to 990.0mL. Mix

thoroughly. Gently heat while stirring and bring to

boiling. Autoclave for 15 min at 15 psi pressure–

121°C. Aseptically add 10.0mL of sterile glutamine/

tryptophan solution. Mix thoroughly. Pour into ster

ile Petri dishes or distribute into sterile tubes.

Use: For the cultivation of Escherichia coli.