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Gluconate Peptone Broth

[所属分类:培养基配方] [发布时间:2021-11-19] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Potassium gluconate .........................................40.0g

Casein peptone....................................................1.5g

K2HPO4...............................................................1.0g

Yeast extract........................................................1.0g

pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Distribute into tubes or flasks. Auto

clave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and differentiation of Gram

negative bacteria based on their ability to oxidize

gluconate to 2-ketogluconate. For the differentiation

of fluorescent Pseudomonas species. After inocula

tion with bacteria and 48 hr of growth in this medi

um, Benedict’s reagent is added. Bacteria that

produce the reducing sugar 2-ketogluconate turn the

reagent yellow-orange to orange-red.