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Gelatinase Test Medium

[所属分类:培养基配方] [发布时间:2021-11-19] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Gelatin.................................................................3.0g

ACES buffer .......................................................1.0g

Yeast extract........................................................1.0g

Charcoal, activated ...........................................0.15g

α-Ketoglutarate monopotassium salt..................0.1g

L-Cysteine·HCl·H2O (4% solution)................. 1.0mL

KOH (85% solution)....................................... 1.0mL

Fe4(P2O7)3 solution......................................... 1.0mL

pH 6.9 ± 0.2 at 25°C

L-Cysteine·HCl·H2O Solution:

Composition per 10.0mL:

L-Cysteine·HCl·H2O............................................0.4g

Preparation of L-Cysteine·HCl·H2O Solu

tion: Add L-cysteine·HCl·H2O to distilled/deionized

water and bring volume to 10.0mL. Mix thoroughly.

Filter sterilize.

Fe4(P2O7)3 Solution:

Composition per 10.0mL:

Fe4(P2O7)3.........................................................0.15g

Preparation of Fe4(P2O7)3 Solution: Add

Fe4(P2O7)3 to distilled/deionized water and bring

volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add ACES buffer to

distilled/deionized water and bring volume to

899.0mL. Mix thoroughly. Gently heat to 50°C. Add

1.0mL of KOH solution. Mix thoroughly. Add char

coal, yeast extract, and α-ketoglutarate. Add 80.0mL

of distilled/deionized water to wash sides of flask.

Mix thoroughly. Autoclave for 15 min at 15 psi pres-

sure–121°C. Cool to 50°C. Aseptically add 10.0mL

of sterile cysteine solution and 10.0mL of sterile

Fe4(P2O7)3 solution. Mix thoroughly. Adjust pH to

6.9. Aseptically distribute into sterile screw-capped

tubes.

Use: For the cultivation and differentiation of gelati

nase-producing bacteria.