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Fluid Thioglycolate Medium

[所属分类:培养基配方] [发布时间:2021-11-19] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein............................... 15.0g

Glucose .............................................................. 5.5g

Yeast extract....................................................... 5.0g

NaCl................................................................... 2.5g

Agar ................................................................. 0.75g

L-Cystine ............................................................ 0.5g

Sodium thioglycolate......................................... 0.5g

Resazurin ........................................................1.0mg

pH 7.1 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Distribute into tubes or flasks. Autoclave for 15 min

at 15 psi pressure–121°C. If medium becomes oxi

dized before use (resazurin turns red), heat in a boil

ing water bath to expel absorbed O2. Cool to 25°C.

Use: For the cultivation of anaerobic, microaero

philic, and aerobic microorganisms. For use in steril

ity testing of a variety of specimens.